Metabolic Phenotyping Facility
Storrs
Department of Nutritional Sciences
The Metabolic Phenotyping Facility has capacities to assess cell and mouse bioenergetics, mouse body composition, and blood lipids, cytokines, and others.
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CLAMS (ABL animal facility 149)
Metabolic cages (Oxymax CLAMS) are used in awake mice to measure energy expenditure, physical activity, indirect calorimetry, and food/water intake. The experiment non-invasively measures O2 consumption and CO2 production in individual mice using metabolic chambers and calculates the respiratory exchange ratio to reflect energy expenditure. Normally, mice are put in a CLAMS metabolic cage for 3 days.
EchoMRI-100 (ABL animal facility 149)
This equipment is a body composition analyzer for small animals (body weight <100 g) that can measure whole body fat, lean, free water, and total water masses in live animals.
Seahorse XFe24 analyzer (ATL 220)
This analyzer determines rates of cellular energy metabolism, e.g., glycolysis and mitochondrial respiration.
The first time user should use “assisted service” for training until he/she can run the assay his/her own.
