JAX Single Cell Biology Laboratory (SCBL)
UConn Health
UConn Health Center Research Advisory Council Cores
The Single Cell Biology Laboratory (SCBL) is a multidisciplinary, research-driven, and collaborative core service specializing in technologies to study cell and tissue biology at single-cell resolution. The SCBL strives to develop, validate, and make available state-of-the-art single-cell and spatial multi-omics technologies. Our staff are highly experienced in tissue dissociation and single cell isolation; cell capture, enrichment; molecular characterization; and the computational analysis of single-cell data sets.
Facility HomepageContacts
General Contact
SCBLservice@jax.orgCampus Address
JAX Single Cell Biology Laboratory
UConn Health Center, Farmington
Mailing Address
10 Discovery Drive
Farmington, CT 06032
Resources
Chromium System (10X Genomics Inc)
A droplet-based single cell RNA-seq system capable of running 8 samples in parallel and generating ~6,000 single cell transcriptomes per sample. More details can be found here.
Drop-Seq Experiment Instrumentation
The lab has all necessary equipment (syringe pumps, microscope) to successfully run Drop-seq experiments as described in Macosko et al. (2015). Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell, 161:1202.
Helios™ Mass Cytometer, a CyTOF System (Fluidigm)
Helios™ is the latest generation (3rd) of the CyTOF® mass cytometer system. It is designed to analyze metal-tagged antibodies in cells suspended in solution (similar to a flow cytometer) but can detect up to 35-40 epitopes simultaneously.
Hyperion™ Laser Ablation Module (Fluidigm)
The Hyperion™ is a separate laser ablation module that can be connected to Helios™ to analyze metal-tagged antibodies in tissue. This, in essence, provides a highly multiplexed (up to 37 antibodies) immunohistochemistry platform with a resolution of 1 um. The first publication to describe this approach is Giesen et al. (2014). Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nature Methods, 11:417.
C1 Single-Cell Auto Prep System (Fluidigm)
A microfluidic chip system that captures individual cells (up to 96 in parallel) and permits automated processing through a series of on-chip molecular steps. Can be used in single cell RNA-seq, single cell ATAC-seq, and other multi-omic approaches.
BioMark HD System (Fluidigm)
A high throughput real-time PCR system amenable to use in single cell studies.
ABSL-2 Cell/Tissue Culture
Equipped for cell dissociations on site prior to analysis by single cell approaches.
Echo 525 Liquid Handler (Labcyte)
A liquid handler that moves liquid by sound (acoustic liquid handling) in 25 nl droplets thus greatly reducing the volumes required in various single cell library preparation protocols.
