Flow Cytometry

Storrs

Center for Open Research Resources and Equipment

Flow Cytometry Facility provides investigators with our expertise and sophisticated instruments for the analysis of cells or particles based on size, internal complexity, and fluorescent signals. Cytometers allow simultaneous analysis of multiple properties of cells or particles and provide information about both single cells and populations. The heterogeneous cells and populations can be identified and quantified. Selected cells or particles can also be physically sorted to obtain pure populations for further analysis or subsequent culture and functional study.

Cytek Aurora CS

The Cytek Aurora CS redefines cell sorting with full-spectrum flow cytometry technology. The Aurora CS offers unparalleled flexibility in panel design and the ability to resolve complex populations with exceptional clarity. Equipped with up to 5 lasers and capable of detecting over 64 parameters, this advanced sorter supports high-speed, high-purity sorting. It is equipped to sort up to 6 separate populations simultaneously, into tubes or plates. With intuitive software, a customizable optical array, and minimal compensation requirements, the Aurora CS is ideal for any sorting need.

BD LSRFortessa X-20 Cell Analyzer

The BD LSRFortessa X-20 flow cytometer, equipped with the High Throughput Sampler (HTS) module, delivers high-parameter flow cytometry with exceptional speed and flexibility. It houses 5 lasers and the ability to detect up to 20 parameters simultaneously. It provides high-resolution and multiparameter analysis suited for complex cell populations. The integrated HTS system enables automated, rapid sample acquisition from 96-well plates, making it ideal for high-throughput screening and large-scale experiments. Engineered for performance, the Fortessa X-20 streamlines data collection without compromising sensitivity or data quality. Independent user status is available. Training is required to become an independent user on the Fortessa X-20. To be trained please complete the training request from. Once trained, the cytometer is available 24 hrs/7 days a week.

Cytek Cloud™

Cytek Cloud™ is a secure, web-based platform designed to streamline and enhance your flow cytometry workflow. Cytek Cloud allows users to upload, manage, and view spectral cytometry data from anywhere. With integrated analysis tools, collaboration features, and direct compatibility with Cytek instruments, it empowers teams to work more efficiently and accelerate discovery. Whether you're in the lab or working remotely, Cytek Cloud keeps your data accessible, organized, and ready for insight. Contact the facility for the instrument serial number.

https://cloud.cytekbio.com/newlogin

Services and Rates

Training and education on flow cytometry theory and facility instruments (required for all new flow users).
Un-assisted acquisition (24/7 access to Fortessa Analyzer, and Analysis Stations for independent users)
Consultations on experimental design and new strategy development
Staff-assisted flow acquisition and data analysis; Cytometric Beads Assay, etc.
Project troubleshooting, data interpretation, deep analysis and customized support
Software support, Flowjo, Diva, etc.
Staff-operated Cell Sorting (Monday-Friday, by appointment through facility director)
Contact facility director for consultation, training, sorting schedule, or any other questions.

Project Request

Training Request

Center for Open Research Resources and Equipment

Flow Cytometry

Staff

Amanda DelVichio, Ph.D.

Faculty Scientific Advisers

Adam Zweifach, Ph.D.

Andrew Wiemer, Ph.D.

David Goldhamer, Ph.D.

Campus Address

Mailing Address

Instrumentation

Cytek Aurora CS

Internal Rates

External Rates

BD LSRFortessa X-20 Cell Analyzer

Internal Rates

External Rates

FlowJo

Cytek Cloud™

Services and Rates